INTENDED USE

The Specific IgE LIA Kit is intended for the semi-qualitative determination of total IgE and sIgE against common inhalation and food allergens in human serum to support the diagnosis of allergic reactions that cause conjunctivitis, rhinitis or asthma.

ASSAY PRINCIPLE

The test is based on the principle of the line immuno assay (LIA). Specific allergens are applied as lines on a nitrocellulose membrane. The nitrocellulose membrane is blocked to prevent unspecific reactions. During incubation of a strip with diluted patient samples, specific antibodies of class IgE present in the sample will bind to the allergens on the strip. For the detection of the bound antibodies, an alkaline phosphatase labeled anti-human IgE antibody is used. After addition of the substrate solution, the appearance of purple blue lines indicates the existence of antibodies against the respective allergens.

斯德润诊断

Line Immuno Assay (LIA) for the Detection of Specific IgE