Specification

Code	Description
ALD-10-A	AMA M2、LKM1、LC1、SLA、F-Actin、gp210、sp100、SSB、SSA/52kd、CENP B
ALD-9-A	AMA M2、LKM1、LC1、SLA、F-Actin、gp210、sp100、SSA/52kd、CENP B
ALD-8B-A	AMA M2、LKM1、LC1、SLA、F-Actin、gp210、sp100、SSB
ALD-8F-A	AMA M2、LKM1、LC1、SLA、F-Actin、gp210、sp100、SSA/52kd
ALD-8C-A	AMA M2、LKM1、LC1、SLA、gp210、sp100、SSA/52kd、CENP B
ALD-7-A	AMA M2、LKM1、LC1、SLA、gp210、sp100、SSA/52kd
ALD-6-A	AMA M2、LKM1、LC1、SLA、gp210、sp100
ALD-4-A	AMA M2、LKM1、LC1、SSA/52kd

INTENDED USE

The liver LIA is for the qualitative measurement of IgG class antibodies against AMA M2, CENPB, sp100, gp210, LKM-1, LC-1, SLA/LP, SSA/52kd in human serum. The assay is intended for in vitro diagnostic use only as an aid in the diagnosis of autoimmune liver disease.

Autoimmune hepatitis (AIH) types 1-3, primary biliary cirrhosis (PBC), and immune cholangiopathy, regarded as the overlap syndrome between AIH and PBC, are some of the most common autoimmune liver diseases. Autoimmune hepatitis is a relatively rare disease. It is necessary to distinguish it from viral hepatitis strictly. Because of the improvement of the serological diagnosis ability, the detection rate of the autoimmune hepatitis also increases. One of the causes is the autoimmune response to liver tissue caused by the decline of the autoimmune tolerance. It is not fully understood that whether the autoantibodies generated in this autoimmune response have pathologic correlation. Perhaps, liver cell damage is caused by T cells.

Autoantibodies play an important role in distinguishing viral hepatitis from three different types of autoimmune chronic active hepatitis (AIH). In AIH type I, ANA and anti smooth muscle antibodies (ASMA) can be detected. In AIH type II, the corresponding antigen is liver and kidney microsome antigen (LKM - 1). Soluble liver antigen antibody mainly appears in AIH type III.

Autoantibodies which could be detected in the corresponding diseases are as below.

AIH type I: ANA, ASMA

AIH type II: LKM-1, LC-1

AIH type III: SLA/LP

PBC: AMA M2, sp100, gp210, (CENP B)

PRINCIPLE

The test is based on the principle of the line immune assay (LIA).

Autoimmune liver diseases related antigens are applied as lines on a nitrocellulose membrane. The nitrocellulose membrane is blocked to prevent unspecific reactions. During incubation of a strip with diluted patient samples, autoantibodies present in the sample will bind to the antigens on the strip. For the detection of the bound antibodies, an alkaline phosphatase labelled antihuman IgG antibody is used. After addition of the substrate solution, the appearance of purple blue lines indicates the existence of (auto) antibodies against the respective antigens.

斯德润诊断

Line Immuno Assay (LIA) for the Detection of Autoantibodies in Autoimmune Liver Diseases